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| Oak infected with Bacterial Leaf Scorch (BLS) |
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| Tree samples ready for Bacterial Leaf Scorch testing at the U of I Plant Clinic. |
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| Cutting petioles for testing them and carefully weighing out each stem sample. |
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| Leaf petiole sample and buffer are added to the bag and ready for grinding within the mesh sample bag. |
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| Loading the test wells for BLS testing. |
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| Added the control buffer to the wells....now time to incubate! |
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| Incubating for 2 hours! |
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| Time to rinse out the wells (8 times). |
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| Making the conjugate to be added to the wells. |
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| Filling the wells with enzyme conjugate and letting incubate again |
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| Incubating again for 2 hours. |
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| Second rinse (8 times) |
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| Add substrate solution and the wait up to 20 (+) minutes. |
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| The blue color indicates a positive result or presence of the pathogen in a particular sample. |
